Investigation on the stability in plant metabolomics with a special focus on freeze-thaw cycles: LC–MS and NMR analysis to Cassiae Semen (Cassia obtusifolia L.) seeds as a case study

化学 代谢组学 色谱法 食品科学 缬氨酸 脯氨酸 生物化学 氨基酸
作者
Biru Shi,Hui Ding,Liming Wang,Chenxi Wang,Xiaoxuan Tian,Zhifei Fu,Lihua Zhang,Lifeng Han
出处
期刊:Journal of Pharmaceutical and Biomedical Analysis [Elsevier]
卷期号:204: 114243-114243 被引量:11
标识
DOI:10.1016/j.jpba.2021.114243
摘要

Metabolomics is a rapid and sensitive tool for the detection of dynamic metabolic compositions in the study of systemic metabolic consequences. However, it is also susceptible to a tiny variation of pre-analytical handling procedures. To provide reproducible results, specific knowledge on metabolites perturbance along with different freeze-thaw cycles (FTCs) is needed for further metabolomics studies. In this paper, five FTCs of germinated Cassiae Semen (CS) were chosen as a case study to investigate the influence of FTC effect based on UHPLC-Q-Orbitrap-MS and NMR technologies. A total of 108 metabolites were relatively quantified by LC–MS and NMR analyses. Principal component analysis (PCA) showed that the first and second FTC samples are welly separated from the other groups; however, the extent of FTC-induced effects are smaller after the third cycle. Upon five consecutive FTCs, alterations which consisted of decreased stachyose, sucrose, norrubrofusarin-6-O-β-d-glucopyranoside, and quercetin 3-(3″-acetylgalactoside), as well as increased phenylalanine, leucine, isoleucine, methionine, phenylalanine, mannose, gluconic acid, and valine, could be observed. FTC does not exert the same effect on all metabolites. Although a large number of secondary metabolites were stable when subjected to five FTCs, FTC effects may lead to false-positive in the discovery of biomarker. In the case of reusing plant seed samples, no more than three consecutive freeze-thaw cycles were found advisable. This work provides unique perspectives on the FTC effects, which may fill in some existing gaps in the knowledge of the stability of plant metabolites during sample pre-handling.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
大模型应助HJJHJH采纳,获得30
1秒前
动听的夜发布了新的文献求助10
4秒前
辉hui发布了新的文献求助10
5秒前
5秒前
luckbaby完成签到,获得积分10
6秒前
7秒前
7秒前
研友_LJGoXn发布了新的文献求助10
10秒前
tengyi完成签到,获得积分10
11秒前
周老八发布了新的文献求助10
12秒前
穿堂风发布了新的文献求助10
12秒前
鲜于夜白完成签到 ,获得积分10
13秒前
科研通AI2S应助当当采纳,获得10
13秒前
14秒前
15秒前
帅气凝云完成签到 ,获得积分10
17秒前
今后应助鸿宇采纳,获得10
17秒前
科研通AI5应助Betsy采纳,获得10
19秒前
共享精神应助LeiX采纳,获得10
20秒前
醍醐不醒完成签到 ,获得积分10
20秒前
26秒前
26秒前
27秒前
29秒前
鸿宇发布了新的文献求助10
30秒前
30秒前
衣裳薄完成签到,获得积分10
31秒前
星星泡饭完成签到,获得积分10
31秒前
32秒前
yyyy发布了新的文献求助10
32秒前
XJYXJY完成签到,获得积分10
32秒前
32秒前
32秒前
当当发布了新的文献求助10
33秒前
34秒前
LeiX发布了新的文献求助10
34秒前
35秒前
35秒前
柚子完成签到,获得积分20
36秒前
yhz_zjut_suda发布了新的文献求助10
37秒前
高分求助中
Continuum thermodynamics and material modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Healthcare Finance: Modern Financial Analysis for Accelerating Biomedical Innovation 2000
Applications of Emerging Nanomaterials and Nanotechnology 1111
Unseen Mendieta: The Unpublished Works of Ana Mendieta 1000
Les Mantodea de Guyane Insecta, Polyneoptera 1000
工业结晶技术 880
热门求助领域 (近24小时)
化学 医学 材料科学 生物 工程类 有机化学 生物化学 纳米技术 内科学 物理 化学工程 计算机科学 复合材料 基因 遗传学 物理化学 催化作用 细胞生物学 免疫学 电极
热门帖子
关注 科研通微信公众号,转发送积分 3491062
求助须知:如何正确求助?哪些是违规求助? 3077779
关于积分的说明 9150152
捐赠科研通 2770160
什么是DOI,文献DOI怎么找? 1520088
邀请新用户注册赠送积分活动 704504
科研通“疑难数据库(出版商)”最低求助积分说明 702196