胞浆
细胞穿透肽
内吞作用
渗透(战争)
肽
细胞生物学
化学
内化
肽序列
小窝
细胞
生物化学
生物
酶
基因
工程类
运筹学
作者
Akiko Okuda,Shiroh Futaki
出处
期刊:Methods in molecular biology
日期:2021-11-12
卷期号:: 265-273
被引量:6
标识
DOI:10.1007/978-1-0716-1752-6_18
摘要
Pas2r12 is comprised of a repeat of the penetration-accelerating sequence (Pas) (Pas2: FFLIG-FFLIG) and D-form dodeca-arginine (r12), a cell-penetrating peptide. Pas2r12 significantly enhances cytosolic delivery of cargo proteins, including enhanced green fluorescent protein and immunoglobulin G. Simply incubating Pas2r12 with cargo leads to their cytosolic tranlsocation. Cytosolic delivery of cargo by Pas2r12 involves caveolae-mediated endocytosis. In this chapter, we describe methods of cytosolic delivery of cargo using Pas2r12 and provide methods for investigating the cellular uptake pathway of cargo by Pas2r12.
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