毛细管电泳
色谱法
衍生化
单糖
化学
银杏
检出限
电泳
萃取(化学)
分析化学(期刊)
高效液相色谱法
有机化学
医学
药理学
作者
Shuo Liu,Xin Li,Xifeng Yang,Linhui Zhou,Xia Liang,Qiu Ru-chen,Yun Fa
标识
DOI:10.1002/jssc.202100749
摘要
A method for the simultaneous determination of six monosaccharides by pre-column derivatization with 1-phenyl-3-methyl-5-pyrazolone and capillary electrophoresis was developed in this work. The derivatization (i.e., reaction temperature, capillary electrophoresis duration, and extraction number) and separation (i.e., pH and buffer concentration) conditions for capillary electrophoresis were optimized. Results showed that the limits of detection under optimal conditions were in the range of 0.036-0.35 mg/L with a mean correlation coefficient >0.99. The recoveries were in the range of 87.3-108.49%, and the relative standard deviations of intra- and inter-day variations were in the ranges of 2.2-3.8 and 3.2-5.0%, respectively. The method was successfully applied to the analysis of six free monosaccharides in three types of Ginkgo biloba leaves.
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