生物
NADPH氧化酶
拟南芥
免疫系统
失调
拟南芥
微生物学
突变体
细胞生物学
肠道菌群
活性氧
遗传学
基因
免疫学
作者
Sebastian Pfeilmeier,Gabriella C. Petti,Miriam Bortfeld‐Miller,Benjamin J. Daniel,Christopher M. Field,Shinichi Sunagawa,Julia A. Vorholt
出处
期刊:Nature microbiology
日期:2021-06-30
卷期号:6 (7): 852-864
被引量:100
标识
DOI:10.1038/s41564-021-00929-5
摘要
The plant microbiota consists of a multitude of microorganisms that can affect plant health and fitness. However, it is currently unclear how the plant shapes its leaf microbiota and what role the plant immune system plays in this process. Here, we evaluated Arabidopsis thaliana mutants with defects in different parts of the immune system for an altered bacterial community assembly using a gnotobiotic system. While higher-order mutants in receptors that recognize microbial features and in defence hormone signalling showed substantial microbial community alterations, the absence of the plant NADPH oxidase RBOHD caused the most pronounced change in the composition of the leaf microbiota. The rbohD knockout resulted in an enrichment of specific bacteria. Among these, we identified Xanthomonas strains as opportunistic pathogens that colonized wild-type plants asymptomatically but caused disease in rbohD knockout plants. Strain dropout experiments revealed that the lack of RBOHD unlocks the pathogenicity of individual microbiota members driving dysbiosis in rbohD knockout plants. For full protection, healthy plants require both a functional immune system and a microbial community. Our results show that the NADPH oxidase RBOHD is essential for microbiota homeostasis and emphasizes the importance of the plant immune system in controlling the leaf microbiota.
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