生物发光
化学
计算生物学
突变
功能(生物学)
萤光素酶类
荧光素酶
生物化学
细胞生物学
酶
生物
突变
基因
转染
作者
K A Beregovaya,N. M. Myshkina,T. V. Chepurnykh,Alexey A. Kotlobay,К. В. Пуртов,Valentin N. Petushkov,Н. С. Родионова,Ilia V. Yampolsky
出处
期刊:Doklady Biochemistry and Biophysics
[Springer Nature]
日期:2021-03-10
卷期号:496 (1): 14-17
被引量:1
标识
DOI:10.1134/s1607672921010026
摘要
The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination.
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