人参
选择性反应监测
质谱法
电喷雾电离
蛋白质沉淀
串联质谱法
三级四极质谱仪
原人参二醇
作者
Chao Ma,Qiyan Lin,Yafu Xue,Zhengcai Ju,Gang Deng,Wei Liu,Yuting Sun,Huida Guan,Xuemei Cheng,Changhong Wang
摘要
A rapid ultra-fast liquid chromatography tandem mass spectrometry method was developed and validated to determine ginsenosides Rk1 and Rg5, a pair of isomers, in rat plasma, which was successfully applied to their pharmacokinetic studies. Two ginsenosides were gave to male Sprague-Dawley rats via intragastrical and intravenous routes, respectively, and the impact of double bonds position on the pharmacokinetic features of two ginsenosides were elucidated in rats. Ginsenoside Rg3 was used as internal standard, and ethyl acetate was applied to extract analytes and internal standard. Chromatographic separation was carried out on a reverse-phase UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm). The flow rate was set to 0.4 mL/min. Fragmentation transition was m/z 765.4 → m/z 101.1 for two ginsenosides. The mobile phases were composed of 0.1% formic acid aqueous solution and acetonitrile. The linear range was 2-1000 ng/mL for two ginsenosides. Intra- and inter-day precisions were less than 11.67%, and accuracy fluctuated from -7.44% to 6.78% for two ginsenosides. The extraction recovery, matrix effect, and stability of two ginsenosides were within acceptable levels. After treatment with ginsenosides Rk1 and Rg5, respectively, some differences were found in their pharmacokinetic profiles in rats. The maximum plasma drug concentration and the area under the plasma drug concentration-time curve of ginsenoside Rg5 were about 5 times bigger than that of ginsenoside Rk1 after oral administration, and 3 times higher after intravenous administration. The oral bioavailabilities of ginsenosides Rk1 and Rg5 were 0.67% and 0.97%, respectively. The results indicated that ∆20(22) -ginsenosides showed better pharmacokinetic features than ∆20(21) -ginsenosides with the same glycosylation.
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