机制(生物学)
细胞外小泡
细胞生物学
细胞凋亡
小泡
弦(物理)
生物物理学
化学
膜
细胞外
纳米技术
生物
物理
生物化学
材料科学
理论物理学
量子力学
作者
Georgia K. Atkin‐Smith,Rochelle Tixeira,Stephanie Paone,Suresh Mathivanan,Christine Collins,Michael Liem,Katharine J. Goodall,Kodi S. Ravichandran,Mark D. Hulett,Ivan K. H. Poon
摘要
Abstract Disassembly of apoptotic cells into smaller fragments (a form of extracellular vesicle called apoptotic bodies) can facilitate removal of apoptotic debris and intercellular communication. However, the mechanism underpinning this process is unclear. While observing monocytes undergoing apoptosis by time-lapse microscopy, we discovered a new type of membrane protrusion that resembles a ‘beads-on-a-string’ structure. Strikingly, the ‘beads’ are frequently sheared off the ‘string’ to form apoptotic bodies. Generation of apoptotic bodies via this mechanism can facilitate a sorting process and results in the exclusion of nuclear contents from apoptotic bodies. Mechanistically, generation of ‘beads-on-a-string’ protrusion is controlled by the level of actomyosin contraction and apoptopodia formation. Furthermore, in an unbiased drug screen, we identified the ability of sertraline (an antidepressant) to block the formation of ‘beads-on-a-string’ protrusions and apoptotic bodies. These data uncover a new mechanism of apoptotic body formation in monocytes and also compounds that can modulate this process.
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