八面体
脂肪酶
材料科学
结晶学
化学工程
晶体结构
化学
有机化学
酶
工程类
作者
Zhiming Chen,Mingsong Wang,Chuanming Zhao,Ying Lin,Renchun Yang,Zhilin Wang
出处
期刊:CrystEngComm
[The Royal Society of Chemistry]
日期:2015-01-01
卷期号:17 (12): 2536-2543
被引量:6
摘要
Fe3O4/CeO2 hybrid octahedra have been successfully synthesized on the large scale via a solvothermal route, which were confirmed by a series of modern analytical tools. The formation and evolution of Fe3O4/CeO2 hybrid octahedra were systematically investigated, and a kinetically induced anisotropic growth mechanism clearly contributes to the formation of the novel architecture. Because of the magnetic nature and unique surfaces, the as-prepared Fe3O4/CeO2 hybrid octahedra were employed as magnetic carriers for lipase immobilization. Our results indicated that the lipase loading amount on the magnetic Fe3O4/CeO2 nanoparticles was about 43.1 mg of protein per g. Hydrolytic activity tests revealed that the immobilized lipase retains about 92.4% of the free enzyme's activity. The reusability assay revealed that the immobilized lipase can be reused 3 times without marked loss of catalytic activity (<5%). Most importantly, the thermal, acid and alkali stabilities of immobilized lipase were strikingly improved. It is hoped that the Fe3O4/CeO2 hybrid octahedra may find applications in biotechnology and biocatalysis.
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