Protein and Peptide Purification

The objective of this chapter is to present a comprehensive overview of the key aspects of downstream process (DSP) development of proteins and peptides. The chapter opens with a general description of the DSP steps and highlights their objectives and the basic bioseparation techniques employed. After introducing the reader to the general aspects of DSP, recent developments in inclusion bodies refolding are presented. There are several challenges facing downstream process development, and strategies for protein/peptide purification process development and how each tries to address/overcome the DSP challenges are reviewed. Finally, expert views on future directions of purification process development efforts are presented. Keywords: downstream processing (DSP); protein purification process development; peptide purification process development; inclusion bodies refolding; bioseparation processing