花生
花生
种质资源
微繁殖
开枪
生物
外植体培养
园艺
腋芽
植物
体外
生物化学
作者
Elif Aylin Özüdoğru,Ergün Kaya,Maurizio Lambardi
出处
期刊:Methods in molecular biology
日期:2012-01-01
卷期号:: 77-87
被引量:4
标识
DOI:10.1007/978-1-62703-074-8_6
摘要
Peanut (Arachis hypogaea L.), also known as groundnut, is the most important species of Arachis genus, originating from Brazil and Peru. Peanut seeds contain high seed oil, proteins, amino acids, and vitamin E, and are consumed worldwide as edible nut, peanut butter, or candy, and peanut oil extracted from the seeds. The meal remaining after oil extraction is also used for animal feed. However, its narrow germplasm base, together with susceptibility to diseases, pathogens, and weeds, decreases yield and seed quality and causes great economic losses annually. Hence, the optimization of efficient in vitro propagation procedures would be highly effective for peanut propagation, as it would raise yield and improve seed quality and flavor. Earlier reports on traditional micropropagation methods, based on axillary bud proliferation which guarantees the multiplication of true-to-type plants, are still limited. This chapter describes a micropropagation protocol to improve multiple shoot formation from shoot-tip explants by using AgNO(3) in combination with plant growth regulators.
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