Microwave assisted green synthesis of fluorescent N-doped carbon dots: Cytotoxicity and bio-imaging applications

细胞毒性 荧光 碳纤维 材料科学 纳米技术 微波食品加热 兴奋剂 化学 光电子学 生物化学 计算机科学 电信 光学 体外 复合数 物理 复合材料
作者
Thomas Nesakumar Jebakumar Immanuel Edison,Raji Atchudan,Mathur Gopalakrishnan Sethuraman,Jae–Jin Shim,Yong Rok Lee
出处
期刊:Journal of Photochemistry and Photobiology B-biology [Elsevier]
卷期号:161: 154-161 被引量:306
标识
DOI:10.1016/j.jphotobiol.2016.05.017
摘要

A fast and facile microwave approach for the synthesis of fluorescent nitrogen-doped carbon dots (N-CDs) is reported. The N-CDs were hydrothermally synthesized using l-ascorbic acid (AA) and β-alanine (BA) as the carbon precursor and the nitrogen dopant, respectively. The morphology of synthesized N-CDs was characterized by high resolution transmission electron microscopy (HR-TEM) and the elemental composition was analyzed using elemental mapping method. The crystallinity and graphitation of N-CDs were examined by X-ray diffraction (XRD) and Raman spectroscopy. The doping of nitrogen over the carbon dots (CDs) was revealed by attenuated total reflection conjunction with Fourier transform infrared (ATR-FTIR) spectroscopy and X-ray photo electron spectroscopy (XPS). The optical properties of synthesized N-CDs were examined by UV–Visible (UV–Vis) and fluorescence spectroscopy. The synthesized N-CDs emit strong blue fluorescence at 401 nm under excitation of 325 nm. The excitation dependent emission property of synthesized N-CDs was exposed from fluorescence results. The quantum yield of synthesized N-CDs is about 14% against the reference quinine sulfate. The cytotoxicity of synthesized N-CDs on Madin-Darby Canine Kidney (MDCK) and HeLa cells were evaluated through Cell Counting Kit-8 (CCK-8) cytotoxicity assay. The results implied that the fluorescent N-CDs showed less cytotoxicity, further which was successfully applied as a staining probe for the confocal imaging of MDCK and HeLa cells.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
矮小的长颈鹿完成签到,获得积分10
1秒前
独特以松发布了新的文献求助10
2秒前
hanghang发布了新的文献求助10
3秒前
可爱的夜阑完成签到 ,获得积分10
5秒前
归海含烟完成签到,获得积分10
5秒前
ZZY发布了新的文献求助10
5秒前
大脸猫完成签到 ,获得积分10
6秒前
研友_LOqqmZ发布了新的文献求助10
7秒前
7秒前
Ll发布了新的文献求助10
7秒前
小蘑菇应助zhy采纳,获得10
8秒前
9秒前
香蕉觅云应助虚拟的绿蕊采纳,获得10
10秒前
Xu发布了新的文献求助10
12秒前
么么么发布了新的文献求助10
12秒前
FashionBoy应助吴彦祖采纳,获得10
15秒前
研友_LOqqmZ完成签到,获得积分10
15秒前
16秒前
17秒前
酷波er应助舒服的基德采纳,获得10
17秒前
cocopepsi完成签到,获得积分10
18秒前
18秒前
18秒前
江峰发布了新的文献求助10
19秒前
21秒前
23秒前
23秒前
ZZY完成签到,获得积分10
23秒前
23秒前
24秒前
25秒前
月亮发布了新的文献求助10
25秒前
笨笨歌曲完成签到,获得积分10
25秒前
25秒前
kk完成签到,获得积分20
26秒前
thinking完成签到,获得积分10
27秒前
you发布了新的文献求助30
27秒前
初妍完成签到,获得积分10
27秒前
28秒前
Owen应助cctv18采纳,获得10
29秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Very-high-order BVD Schemes Using β-variable THINC Method 830
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3247691
求助须知:如何正确求助?哪些是违规求助? 2890959
关于积分的说明 8265537
捐赠科研通 2559214
什么是DOI,文献DOI怎么找? 1387979
科研通“疑难数据库(出版商)”最低求助积分说明 650670
邀请新用户注册赠送积分活动 627552