Studies on Chemical Constituents of Paecilomyces hepiali

The chemical constituents of Paecilomyces hepialiwere studied,and the chemicals were separated and purified by gradient elution,repeated recrystallization and silica gel chromatography.NMR spectroscopic was used to elucidate their structures.Six compounds were separated from Paecilomyces hepiali,which was 4,6,8(14),22(23)-tetraen-3-one-ergostane(1),ergosterol(2),β-sitosterol(3),ergosterol peroxide(4),Beer sterol(5),mannitol(6),1-oleoyl-2-linoleic-3-palmitic acid glycerol(7).In addition,it took GC-MS on the petroleum ether extract oily component,and the main compound was linoleic acid(9,12-Octadecadienoic acid),the content of which was 41.949%,9-Octadecadienoic acid,the content of which was 27.696%;And using High Performance Liquid Chromatography method to determine ergosterol,which was compared with Paecilomyces hepiali,Pleurotus ostreatus,Pleurtus eryngi,Lentinula edodes,Flammulina velutipes,Pholiota microspora,Hypsizygus marmoreus,the ergosterol content of which were respectively 15.65,21.43,10.16,13.34,9.48,10.43,14.42 mg/g.