细胞毒性
细胞凋亡
p38丝裂原活化蛋白激酶
癌细胞
化学
顺铂
MAPK/ERK通路
活力测定
A549电池
活性氧
细胞培养
细胞生物学
分子生物学
生物
激酶
生物化学
癌症
体外
遗传学
化疗
作者
Chun‐Gon Kim,Verónica Castro-Aceituno,Ragavendran Abbai,Hyun A. Lee,Shakina Yesmin Simu,Yaxi Han,Joon Hurh,Yeon-Ju Kim,Deok Chun Yang
标识
DOI:10.1016/j.biopha.2018.01.050
摘要
Siberian ginseng (Eleutherococcus senticosus) was used for the synthesis of an ecofriendly silver nanoparticle (Sg-AgNP), which has exhibited antibacterial, antioxidant effect and lower cytotoxicity to normal cells in comparison to human cancer cells. Although, the potential anticancer activity of Sg-AgNP has not been determined. In this study, two cancer cell lines were used to evaluate the cytotoxicity and apoptotic effect of Sg-AgNP along with the determination of the role of the Caspase-3 / p38 MAPK pathways. Results shown that Sg-AgNP reduced the cell viability of colon cancer cells HT29 and lung cancer cells A549. The cytotoxic effect was higher than the effect exhibited by a commercial silver nanoparticle and Cisplatin. Reactive oxygen species were observed to be superior in both cell lines in the presence of Sg-AgNPs than c-AgNPs and Cisplatin. It was observed an activation of MAPK14 gene and phosphorylation of p38 MAPK protein in both cell lines induced by Sg-AgNPs treatment. Furthermore, induction of morphological changes in the nucleus was done by Sg-AgNPs at 10 μg/mL in both cell lines. On the other hands, the activation of CASP3 gene and Caspase-3 protein was observed in HT29 cells but only at protein level in A549 cells. These results, suggest that Sg-AgNPs anticancer potential activity might be linked to the induction of apoptosis though the generation of ROS by activation of the Caspase-3/p38 MAPK pathway.
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