单纯疱疹病毒
溶解循环
病毒学
生物
螺旋神经节
病毒潜伏期
病毒
细胞培养
潜伏病毒
分子生物学
病毒复制
内耳
遗传学
解剖
作者
Yuehong Liu,Shufeng Li
出处
期刊:ORL
[S. Karger AG]
日期:2015-01-01
卷期号:77 (3): 141-149
被引量:3
摘要
<b><i>Purpose of the Study:</i></b> Reactivation of latent herpes simplex virus type 1 (HSV-1) in spiral ganglion neurons (SGNs) is supposed to be one of the causes of idiopathic sudden sensorineural hearing loss. This study aims to establish a cell culture model of latent and lytic HSV-1 infection in spiral ganglia. <b><i>Procedures:</i></b> In the presence of acyclovir, primary cultures of SGNs were latently infected with HSV-1 expressing green fluorescent protein. Four days later, these cells were treated with trichostatin A (TSA), a known chemical reactivator of HSV-1. TCID<sub>50</sub> was used to measure the titers of virus in cultures on Vero cells. RNA from cultures was detected for the presence of transcripts of ICP27 and latency-associated transcript (LAT) using reverse transcription polymerase chain reaction. <b><i>Results:</i></b> There is no detectable infectious HSV-1 in latently infected cultures, whereas they could be observed in both lytically infected and latently infected/TSA-treated cultures. LAT was the only detectable transcript during latent infection, whereas lytic ICP27 transcript was detected in lytically infected and latently infected/TSA-treated cultures. <b><i>Conclusion:</i></b> Cultured SGNs can be both latently and lytically infected with HSV-1. Furthermore, latently infected SGNs can be reactivated using TSA, yielding infectious virus.
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