间质细胞
CD38
骨髓
等离子体电池
CD19
生物
细胞培养
细胞生物学
细胞分化
CD40
分子生物学
免疫学
抗体
癌症研究
体外
干细胞
细胞毒性T细胞
川地34
生物化学
遗传学
基因
作者
Michio Kawano,Keiichiro Mihara,Naihui Huang,Takako Tsujimoto,Atsushi Kuramoto
出处
期刊:Blood
[American Society of Hematology]
日期:1995-01-15
卷期号:85 (2): 487-494
被引量:78
标识
DOI:10.1182/blood.v85.2.487.bloodjournal852487
摘要
The bone marrow (BM) is well known to be the major site of Ig production in secondary immune responses; thus, the microenvironment of BM is considered to be essential for final differentiation of plasma cells. We identified in the peripheral blood (PB) early plasma cells (CD38++CD19+VLA-5-) committed to entering the BM. The sorted early plasma cells rapidly entered apoptosis in vitro, but these cells could survive and further differentiate into mature plasma cells (CD38 CD19+) just as BM plasma cells in the presence of a BM-derived stromal cell line (KM-102). Culture supernatants of KM-102 cell lines could also support survival of these cells, and antibody to interleukin-6 (IL-6) completely blocked the effect of these supernatants. Furthermore, recombinant IL-6, but not IL-1 or IL-3, could support their survival and their differentiation into mature plasma cells (CD38 CD19+VLA-5+) with expression of VLA-5 mRNA. Therefore, here is direct evidence that early plasma cells found in the PB differentiated into mature plasma cells with stromal cell-derived IL-6 in vitro; thus, BM stromal cells control the final checkpoint of plasma cell differentiation with secretion of IL-6 in the BM.
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