间皮细胞
腹膜透析
连续不卧床腹膜透析
腹膜液
腹膜
碱性成纤维细胞生长因子
间皮
白蛋白
碳酸氢盐
内科学
内分泌学
医学
活力测定
牛血清白蛋白
生长因子
化学
免疫学
生物化学
病理
细胞
受体
作者
Satoshi Ogata,Noriaki Yorioka,Kei Kiribayashi,TAKAYUKI NAITÔ,Masatoshi Kuratsune,Yoji Nishida
出处
期刊:PubMed
日期:2003-01-01
卷期号:19: 2-5
被引量:7
摘要
In patients on long-term continuous ambulatory peritoneal dialysis (CAPD), peritoneal dysfunction is considered to be due to the loss of peritoneal mesothelial cells and to subsequent peritoneal fibrosis and neovascularization. Our aim in the present study was to clarify the role of various components of peritoneal dialysis fluid in the occurrence of peritoneal dysfunction in CAPD patients. We used a cell counting assay and ELISA to study the viability of human peritoneal mesothelial cells and their secretion of basic fibroblast growth factor (bFGF)--which induces peritoneal fibrosis and neovascularization--by cells cultured with various components of peritoneal dialysis fluid. The viability of cultured cells, ranked from highest to lowest by solution type, was bicarbonate (40 mEq/L) > lactate (15 mEq/L) + bicarbonate (25 mEq/L) > lactate (40 mEq/L). Viability also showed a concentration-dependent decrease in the presence of advanced glycation end-products of bovine serum albumin. The bFGF level in the supernatant showed a concentration-dependent increase in the presence of glucose and glycated albumin; bFGF level decreased as the bicarbonate concentration increased. Low levels of glucose, lactate, and glycated albumin, and a high concentration of bicarbonate may preserve the viability of peritoneal mesothelial cells and prevent bFGF secretion.
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