操纵子
双组分调节系统
支原体
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生物
基因
编码区
遗传学
发起人
共转运蛋白
转录调控
生物化学
基因表达
运输机
肽序列
结核分枝杆菌
大肠杆菌
肺结核
医学
突变体
病理
作者
Maria Kanwal Ali,Xinfeng Li,Qing Tang,Xiaoyu Liu,Fang Chen,Jinfeng Xiao,Muhammad Ali,Shan‐Ho Chou,Jin He
标识
DOI:10.3389/fmicb.2017.00570
摘要
Kdp-ATPase is an inducible high affinity potassium uptake system that is widely distributed in bacteria, and is generally regulated by the KdpD/KdpE two-component system. In this study, conducted on Mycobacterium smegmatis, the kdpFABC expression was found to be affected by low concentration of K+, high concentrations of Na+, and/or NH4+ of the medium. The KdpE was found to be a transcriptional regulator that bound to a specific 22-bp sequence in the promoter region of kdpFABC operon to positively regulate kdpFABC expression. The KdpE binding motif was highly conserved in the promoters of kdpFABC among the mycobacterial species. 5'-RACE data indicated a transcriptional start site of the kdpFABC operon within the coding sequence of MSMEG_5391, which comprised a 120-bp long 5'-UTR and an open reading frame of the 87-bp kdpF gene. The kdpE deletion resulted in the altered growth rate under normal and low K+ conditions. Furthermore, under K+ limiting conditions, a single transcript (kdpFABCDE) spanning both kdpFABC and kdpDE operons was observed. This study provided the first insight into the regulation of kdpFABC operon by the KdpD/KdpE two-component system in M. smegmatis.
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