蛋白质-蛋白质相互作用
化学
小分子
泛素
内德4
生物化学
计算生物学
生物
泛素连接酶
基因
作者
Sisley Austin,Saı̈d Taouji,Éric Chevet,Harald Wodrich,Fabienne Rayne
出处
期刊:Methods in molecular biology
日期:2016-01-01
卷期号:: 453-467
被引量:3
标识
DOI:10.1007/978-1-4939-3756-1_30
摘要
AlphaScreen® is a technology particularly suitable for bi-molecular inhibitor screening assays, e.g. using protein–protein interactions with purified recombinant proteins. Each binding partner of the bi-molecular interaction is coupled either to donor or to acceptor beads. The technology is based on the quantifiable transfer of oxygen singlets from donor to acceptor microbeads brought together by a specific interaction between the partners. We identified the conserved interaction between WW domains of cellular ubiquitin ligases of the Nedd4 family and a short peptide motif (PPxY) present in several structural and non-structural viral proteins as a potential drug target. Using an AlphaScreen assay recapitulating the interaction between Nedd4.2 and the PPxY motif of the adenoviral capsid protein VI, we screened a library of small molecules and identified specific inhibitors of this interaction.
科研通智能强力驱动
Strongly Powered by AbleSci AI