活性污泥
化学
蒸馏水
纤维素酶
羟基烷酸
肥料
制浆造纸工业
有机质
废水
丙酮
稻草
水解
脂肪酶
色谱法
废物管理
酶
有机化学
无机化学
细菌
工程类
生物
遗传学
作者
He Ni,Xiaomin Fan,Hao-Ning Guo,Liang Jianhua,Qingrong Li,Yang Liu,Hui Li,Haihang Li
标识
DOI:10.1080/10826068.2017.1286599
摘要
The urban wastewater treatment industry produces a large amount of excess activated sludge which is mainly composed of microbial biomass and costly to be disposed. In this research, a comprehensive utilization of activated sludge was developed by sequentially extracting hydrolytic enzymes and polyhydroxyalkanoates (PHAs), and the residue was used to prepare water-retaining organic fertilizer. The sludge was extracted with fourfold H2O-containing 1% Triton X-100 with the yield of 66.7% protease activity. The enzyme solution was precipitated in 80% acetone and vacuum dried at 40°C at the dried enzyme yield of 2.4 g/kg wet sludge. The enzyme product contains collagenase, lipase, amylase, and cellulase activities, which are good compound enzymes to feed. The PHAs were extracted with 30% sodium hypoclorite:chloroform (1:3). The PHA solution was decolored and dried, and pure white PHAs were obtained at the yield of 70.1 g/kg wet sludge. The residue was used to prepare water-retaining organic fertilizer at the optimal condition. The fertilizer absorbs 131.3-fold distilled water and had good performance in water retention and can effectively slow down the loss of soil moisture when added into soil. This work provides a simple and practical approach for comprehensive utilizing activated sludge with significant economic benefits.
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