Comprehensive utilization of activated sludge for the preparation of hydrolytic enzymes, polyhydroxyalkanoates, and water-retaining organic fertilizer

活性污泥 化学 蒸馏水 纤维素酶 羟基烷酸 肥料 制浆造纸工业 有机质 废水 丙酮 稻草 水解 脂肪酶 色谱法 废物管理 有机化学 无机化学 细菌 工程类 生物 遗传学
作者
He Ni,Xiaomin Fan,Hao-Ning Guo,Liang Jianhua,Qingrong Li,Yang Liu,Hui Li,Haihang Li
出处
期刊:Preparative Biochemistry & Biotechnology [Informa]
卷期号:47 (6): 611-618 被引量:17
标识
DOI:10.1080/10826068.2017.1286599
摘要

The urban wastewater treatment industry produces a large amount of excess activated sludge which is mainly composed of microbial biomass and costly to be disposed. In this research, a comprehensive utilization of activated sludge was developed by sequentially extracting hydrolytic enzymes and polyhydroxyalkanoates (PHAs), and the residue was used to prepare water-retaining organic fertilizer. The sludge was extracted with fourfold H2O-containing 1% Triton X-100 with the yield of 66.7% protease activity. The enzyme solution was precipitated in 80% acetone and vacuum dried at 40°C at the dried enzyme yield of 2.4 g/kg wet sludge. The enzyme product contains collagenase, lipase, amylase, and cellulase activities, which are good compound enzymes to feed. The PHAs were extracted with 30% sodium hypoclorite:chloroform (1:3). The PHA solution was decolored and dried, and pure white PHAs were obtained at the yield of 70.1 g/kg wet sludge. The residue was used to prepare water-retaining organic fertilizer at the optimal condition. The fertilizer absorbs 131.3-fold distilled water and had good performance in water retention and can effectively slow down the loss of soil moisture when added into soil. This work provides a simple and practical approach for comprehensive utilizing activated sludge with significant economic benefits.
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