Soil is a complex and dynamic ecosystem that is home to a large number of microbial communities. These communities are composed of diverse groups of organisms, including bacteria, fungi, archaea, and protists. Among these, protists are the least studied and recognized organisms in the soil ecosystem, yet they play a significant role in maintaining soil health and ecosystem functioning. Despite their ecological importance, soil protists are often understudied, and relatively little is known about their diversity, distribution, and ecological functions. Recent advances in molecular techniques have helped to shed light on the diversity and role of soil protists in ecosystem processes, and further research in this area may have important implications for the maintenance of soil health and biodiversity. However, the initial observation of soil protists can give a basic idea of the diversity and abundance of the organisms present in the soil. Investigating soil protists is an essential step toward understanding and managing soil ecosystems, and it provides important insights into the complex interactions between microorganisms and soil nutrients. Therefore, the aim of this chapter is to provide an overview of initial observation of soil protists. Generally, two different methods are used to observe naked amoeba and active forms of ciliates and flagellates.