表面改性
多酚
对偶(语法数字)
组合化学
化学
抗菌活性
纳米技术
材料科学
有机化学
细菌
艺术
抗氧化剂
生物
文学类
物理化学
遗传学
作者
Xiao Chen,Meizhou Sun,Lujiao Zhang,Yang Hu,Zhiwei Yang,Shun Duan,Fu‐Jian Xu,Juehua Jing
标识
DOI:10.1016/j.cej.2024.151792
摘要
Infected bone defect is a critical problem in clinical practice of orthopedics. To solve this problem, implants with dual-functional surfaces which possess both antibacterial and osteogenic properties have extensive applications. However, there remains a pressing need to develop a convenient strategy for preparing such surfaces facilely. In this study, we reported a novel one-step polyphenol-based surface functionalization strategy for preparing two fibrous membranes with antibacterial and osteogenic capabilities. The formation of RGD-HHC36 (RH) or RGD-polyhexamethylene guanidine (RP) coating was fabricated by Michael reaction with poly (tannic acid) (PTA) to produce dual-functional fibrous membranes (PLA-RH and PLA-RP, respectively). The surfaces of PLA-RH and PLA-RP exhibited increased roughness and hydrophilicity. In vitro tests revealed that PLA-RH and PLA-RP could eradicate over 99% of clinically common pathogenic bacteria while exhibiting significant improvements in the adhesion, proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Moreover, RNA-Seq analysis revealed that the up-regulation of Chrdl1 played a key role in promoting ossification through activation of the CHRDL1-BMP4 pathway. The favourable in vivo anti-infection and osteogenesis-promoting performances were demonstrated in an infected bone defect model. PLA-RH and PLA-RP inhibited bacterial infection in the early stage, and enhanced osteogenesis in the late stage. This study provides a facile and universal strategy for designing and fabricating dual-functional surfaces for antibacterial application and tissue regeneration simultaneously.
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