生物
怀孕
肝细胞
细胞生物学
细胞生长
内分泌学
内科学
遗传学
医学
体外
作者
Shun He,Zhihou Guo,Mingshan Zhou,Haichang Wang,Zhuonan Zhang,Mengyang Shi,Xufeng Li,Xueying Yang,Lingjuan He
出处
期刊:Cell Stem Cell
[Elsevier]
日期:2023-10-03
卷期号:30 (11): 1549-1558.e5
被引量:5
标识
DOI:10.1016/j.stem.2023.09.002
摘要
The maternal liver undergoes dramatic enlargement to adapt to the increased metabolic demands during pregnancy. However, the cellular sources for liver growth during pregnancy remain largely elusive. Here, we employed a proliferation recording system, ProTracer, to examine the spatial-temporal proliferation of hepatocytes during pregnancy. We discovered that during early to late pregnancy, hepatocyte proliferation initiated from zone 1, to zone 2, and lastly to zone 3, with the majority of new hepatocytes being generated in zone 2. Additionally, using single-cell RNA sequencing, we observed that Ccnd1 was highly enriched in zone 2 hepatocytes. We further applied dual-recombinase-mediated genetic lineage tracing to reveal that Ccnd1+ hepatocytes expanded preferentially during pregnancy. Moreover, we demonstrated that estrogen induces liver enlargement during pregnancy, which was abolished in Ccnd1 knockout mice. Our work revealed a unique spatial-temporal hepatocyte proliferation pattern during pregnancy, with Ccnd1+ hepatocytes in zone 2 serving as the major cellular source for hepatic enlargement.
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