松墨天牛
生物
樟脑
寄主(生物学)
米尔辛
冰片
基因敲除
松属
植物
生态学
生物化学
柠檬烯
长角甲虫
医学
细胞凋亡
替代医学
中医药
病理
精油
作者
Ruinan Yang,Dongzhen Li,Shan‐Cheng Yi,Yi Wei,Man‐Qun Wang
标识
DOI:10.1111/1744-7917.13238
摘要
Abstract Monochamus alternatus is the primary carrier of pine wood nematodes, which pose a serious threat to Pinus spp. in many countries. Newly emerging M. alternatus adults feed on heathy host pines, while matured adults transfer to stressed host pines for mating and oviposition. Several odorant‐binding proteins (OBPs) of M. alternatus have been proved to aid in the complex process of host location. To clarify the corresponding relations between OBPs and pine volatiles, more OBPs need to be studied. In this research, MaltOBP19 showed a specific expression in the antennae and mouthparts of M. alternatus , and it was marked in 4 types of antenna sensilla by immunolocalization. Fluorescence binding assays demonstrated the high binding affinity of MaltOBP19 with camphene and myrcene in vitro. In Y‐tube olfactory experiments, M. alternatus adults were attracted by camphene and RNAi of OBP19 via microinjection significantly decreased their attraction index. Myrcene induced phobotaxis, but RNAi had no significant effect on this behavior. Further, we found that ingesting dsOBP19 produced by a bacteria‐expressed system with a newly constructed vector could lead to the knockdown of MaltOBP19. These results suggest that MaltOBP19 may play a role in the process of host conversion via the recognition of camphene, which has been identified to be strongly released in stressed host pines. In addition, it is proved that knockdown of OBP can be achieved by oral administration of bacteria‐expressed double‐stranded RNA in M. alternatus adults, providing a new perspective in the control of M. alternatus .
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