DNA
分子生物学
南方斑点
DNA提取
病毒学
生物
化学
遗传学
基因
聚合酶链反应
作者
Mei Yu,Maura Dandri,Guofeng Cheng,William E. Delaney,Simon P. Fletcher,Lena Allweiss
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 113-124
标识
DOI:10.1007/978-1-0716-4027-2_10
摘要
HBV covalently closed circular DNA (cccDNA) plays an important role in the persistence of hepatitis B virus (HBV) infection by serving as the template for transcription of viral RNAs. To cure HBV infection, it is expected that cccDNA needs either to be eliminated or silenced. Hence, precise cccDNA quantification is essential. Sample preparation is crucial to specifically detect cccDNA. Southern blot is regarded as the "gold standard" for specific cccDNA detection but lacks sensitivity. Here, we describe a rapid and reliable modified kit-based, HBV protein-free DNA extraction method as well as a novel enhanced sensitivity Southern blot that uses branched DNA technology to detect HBV DNA in cell culture and liver tissue samples. It is useful for both HBV molecular biology and antiviral research.
科研通智能强力驱动
Strongly Powered by AbleSci AI