Zinc Finger Protein 536 is a Potential Prognostic Biomarker that Promotes Neuroblastoma Progression via VEGFR2-PI3K-AKT Pathway

PI3K/AKT/mTOR通路 蛋白激酶B 生物标志物 锌指 癌症研究 神经母细胞瘤 血管内皮生长因子受体 医学 信号转导 内科学 生物 细胞生物学 转录因子 生物化学 基因 遗传学 细胞培养
作者
Yuan Fang,Rui Dong,Shujing Wang,Lian Chen,Yizhen Wang,Qiang Wu
出处
期刊:Current Cancer Drug Targets [Bentham Science Publishers]
卷期号:25
标识
DOI:10.2174/0115680096332753240913104409
摘要

Background: Neuroblastoma (NB) is a well-known pediatric malignancy intertwined with neurodevelopment. Previously implicated in neuronal differentiation, Zinc Finger Protein 536 (ZNF536) has emerged as a promising prognostic and immune-related biomarker in our pan-cancer analysis. Methods: Single-cell RNA transcriptome sequencing, bulk transcriptome analysis, and immuno-histochemistry were used to assess ZNF536 expression and its association with prognosis. Cell proliferation, migration, invasion, and differentiation in ZNF536-knockdown NB cell lines were detected to evaluate the effect of ZNF536 on tumor cells. Vascular Endothelial Growth Factor Receptor 2 (VEGFR2), a potential target of ZNF536, and its downstream PI3K/AKT signaling cascade were investigated using transcriptome sequencing, CUT&Tag, quantitative real-time PCR (qRT-PCR), and Western blotting. The role of ZNF536 in tumorigenesis and the potential regulation axis was evaluated in vivo using a BALB/c nude mouse xenograft tumor model. Results: ZNF536 mRNA and protein expression were significantly higher in NB patients with poor prognosis. In vitro, ZNF536 knockdown curtailed proliferation, migration, and invasion of NB cells while fostering differentiation. ZNF536 regulated VEGFR2 expression, thus activating the PI3K-AKT pathway. In vivo, ZNF536 knockdown reduced tumor growth and proliferation via the VEGFR2-PI3K-AKT pathway. Conclusion: ZNF536 resulted as a novel prognostic biomarker in NB, promoting oncogenesis through VEGFR2-PI3K-AKT signaling axis modulation, suggesting its therapeutic potential in managing NB progression.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
耶路生完成签到,获得积分10
1秒前
hyl发布了新的文献求助10
1秒前
2秒前
小猴子完成签到,获得积分20
3秒前
lilililininini完成签到,获得积分20
3秒前
5秒前
6秒前
Ceka完成签到,获得积分10
7秒前
8秒前
8秒前
hyl完成签到,获得积分10
9秒前
积极的初南完成签到,获得积分10
10秒前
Livrik发布了新的文献求助10
11秒前
小小完成签到 ,获得积分10
11秒前
笨笨山芙发布了新的文献求助10
12秒前
13秒前
13秒前
14秒前
14秒前
15秒前
16秒前
量子星尘发布了新的文献求助10
17秒前
Livrik完成签到,获得积分10
17秒前
小猴子关注了科研通微信公众号
17秒前
阿Q完成签到,获得积分10
18秒前
yujd完成签到,获得积分10
18秒前
鹿仪发布了新的文献求助10
18秒前
18秒前
18秒前
Orange应助好宝宝采纳,获得10
20秒前
20秒前
GL发布了新的文献求助10
21秒前
21秒前
李健应助年轻的烨华采纳,获得10
23秒前
Jasper应助zhugepengju采纳,获得10
23秒前
24秒前
酢浆草小熊完成签到 ,获得积分10
24秒前
沈昊泽完成签到,获得积分10
27秒前
陈浩发布了新的文献求助10
27秒前
28秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3988868
求助须知:如何正确求助?哪些是违规求助? 3531255
关于积分的说明 11253071
捐赠科研通 3269858
什么是DOI,文献DOI怎么找? 1804822
邀请新用户注册赠送积分活动 881994
科研通“疑难数据库(出版商)”最低求助积分说明 809035