Effects of Myrcia pubipetala Miq (Myrtaceae) extract on innate inflammatory response

AbstractAbstractMyrcia is a genus widespread in South America with many species presenting anti-inflammatory and biological properties. We investigated the anti-inflammatory activity of crude hydroalcoholic extract of Myrcia pubipetala leaves (CHE-MP) using macrophages (RAW 264.7), and the air pouch model in mice to evaluate leukocyte migration and mediator's release. Adhesion molecule expression, CD49 and CD18, was evaluated in neutrophils. In vitro, the CHE-MP significantly reduced nitric oxide (NO), interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) levels in the exudate and the supernatant culture. CHE-MP did not present cytotoxicity and modulated the percentage of positive neutrophils for CD18 and its expression per cell, without modifying the expression of CD49, which corroborated with significantly reduced neutrophil migration to inflammatory exudate and subcutaneous tissue. Taken together, the data demonstrate that CHE-MP presents a potential activity on innate inflammatory.Graphical AbstractKeywords: Myrcia pubipetalainnate responseinflammationMyrtaceaemacrophagesneutrophil Disclosure statementThe authors declare no conflicts of interest.Additional informationFundingThe authors are grateful to CNPq, FAPESC, UNIEDU, FURB, UNIVALI and UFSC for financial support.