清脆的
计算生物学
基因组工程
生物
工程类
基因组编辑
遗传学
基因
作者
Angelina Job Kolady,Aritra Mukherjee,Ranjith Kumavath,Sarvepalli Vijay Kumar,Pasupuleti Sreenivasa Rao
出处
期刊:BENTHAM SCIENCE PUBLISHERS eBooks
[BENTHAM SCIENCE PUBLISHERS]
日期:2024-02-12
卷期号:: 1-17
标识
DOI:10.2174/9789815165678124010004
摘要
Bacteria is one of the most primitive organisms on earth. Its high susceptibility to bacteriophages has tailored them to use specific tools to edit their genome and evade the bacteriophages. This defense system has been developed to be the most specific genome editing technology of this current period. Previously, various other tools such as restriction enzymes (RE), zinc finger nucleases (ZNF), and transcription activator-like effector nucleases (TALENS) were utilized. Still, its major limitations led to exploiting the bacterial defense system to edit the genome. CRISPR technology can be applied in various microbiology, pathology, cancer biology, molecular biology, and industrial biotechnology, but its limitations, such as off-target effects due to unspecific alterations, are a major concern. In the future, this effective gene alteration technology will be developed to treat inherited rare genetic disorders. This chapter highlights the discovery, components, applications, limitations, and future prospects of CRISPR-Cas.
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