Pleurotus ostreatus polysaccharide-mediated modulation of skin damage caused by microcystin-LR in tadpoles

微生物学 氧化应激 多糖 超氧化物歧化酶 平菇 生物 微囊藻毒素 谷胱甘肽 抗氧化剂 褐藻糖胶 化学 生物化学 食品科学 蘑菇 细菌 蓝藻 遗传学
作者
Yilin Shu,Hui Wang,Huiling Jiang,Shiwen Zhou,Liyuan Zhang,Zifang Ding,Pei Hong,Jun He,Hailong Wu
出处
期刊:Environmental Pollution [Elsevier]
卷期号:345: 123440-123440
标识
DOI:10.1016/j.envpol.2024.123440
摘要

In this study, we aimed to evaluate the effect of dietary supplementation with edible mushroom (Pleurotus ostreatus)-derived polysaccharides on microcystin leucine-arginine (MC-LR)-induced skin damage in Pelophylax nigromaculatus tadpoles. Tadpoles were exposed to 1 μg/L daily MC-LR, with or without 5.0 g/kg of dietary P. ostreatus polysaccharides, for 30 days. P. ostreatus polysaccharide supplementation significantly increased the dermal collagen fibrils, increased tight junction protein gene expression, decreased the amount of MC-LR accumulation in skin tissues, attenuated oxidative stress, downregulated apoptosis-associated gene transcription, decreased eosinophil numbers, and downregulated transcription of inflammation-related genes (e.g. TLR4, NF-κB, and TNF-α). The composition of the skin commensal microbiota of MC-LR-exposed tadpoles supplemented with P. ostreatus polysaccharides was similar to that of the no-treatment control group. Lipopolysaccharide (LPS) content was positively correlated with the abundance of Gram-negative bacteria, including Chryseobacterium and Thauera. Therefore, P. ostreatus polysaccharides may alleviate MC-LR-induced skin barrier damage in tadpoles in two ways: 1) attenuation of oxidative stress-mediated apoptosis mediated by increased glutathione (GSH) content and total superoxide dismutase activity; and 2) alteration of the skin commensal microbiota composition to attenuate the LPS/Toll-like receptor 4 inflammatory pathway response. Furthermore, P. ostreatus polysaccharides may increase skin GSH synthesis by promoting glycine production via the gut microbiota and may restore the MC-LR-damaged skin resistance to pathogenic bacteria by increasing antimicrobial peptide transcripts and lysozyme activity. This study highlights for the first time the potential application of P. ostreatus polysaccharides, an ecologically active substance, in mitigating the skin damage induced by MC-LR exposure, and may provide new insights for its further development in aquaculture.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
充电宝应助嘻嗷采纳,获得10
2秒前
2秒前
脑洞疼应助葭月十七采纳,获得10
2秒前
小二郎应助典雅的俊驰采纳,获得10
2秒前
3秒前
帅气书文发布了新的文献求助10
4秒前
举栗子发布了新的文献求助10
4秒前
4秒前
4秒前
orixero应助简单的冬瓜采纳,获得10
5秒前
MMMMM完成签到,获得积分10
5秒前
情怀应助ypeng采纳,获得10
6秒前
nuantong1shy发布了新的文献求助30
6秒前
打打应助幸福大白采纳,获得10
6秒前
wwwanfg完成签到,获得积分10
7秒前
8秒前
123lx完成签到,获得积分20
8秒前
Clover04应助胡博士采纳,获得10
8秒前
9秒前
真谛发布了新的文献求助10
9秒前
9秒前
MMMMM发布了新的文献求助10
9秒前
菲菲呀发布了新的文献求助10
9秒前
小郭发布了新的文献求助10
11秒前
迷路的芝麻完成签到 ,获得积分10
11秒前
11秒前
12秒前
葭月十七发布了新的文献求助10
13秒前
会飞的猪完成签到,获得积分10
14秒前
15秒前
一口一个小朋友完成签到,获得积分10
15秒前
15秒前
科研真累发布了新的文献求助10
16秒前
boya完成签到,获得积分10
16秒前
三口一头大象应助咕噜噜采纳,获得20
16秒前
奋斗靖仇完成签到 ,获得积分10
17秒前
大个应助闪闪向日葵采纳,获得10
18秒前
Xy完成签到,获得积分10
18秒前
盛夏完成签到,获得积分10
18秒前
友好小之发布了新的文献求助30
19秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Very-high-order BVD Schemes Using β-variable THINC Method 870
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
Fundamentals of Dispersed Multiphase Flows 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3254507
求助须知:如何正确求助?哪些是违规求助? 2896674
关于积分的说明 8293818
捐赠科研通 2565675
什么是DOI,文献DOI怎么找? 1393195
科研通“疑难数据库(出版商)”最低求助积分说明 652443
邀请新用户注册赠送积分活动 630000