拉布
内体
GTP酶
生物
细胞生物学
分泌物
转运蛋白
高尔基体
跨膜蛋白
囊泡转运蛋白
鸟嘌呤核苷酸交换因子
脉络膜缺失
生物化学
小泡
基因
受体
内质网
细胞内
膜
作者
Xinxin Li,Bowen Liu,Yuhan Wen,Jiabin Wang,Yusong R. Guo,Anbing Shi,Long Lin
标识
DOI:10.1083/jcb.202306107
摘要
Multiple physiology-pertinent transmembrane proteins reach the cell surface via the Golgi-bypassing unconventional protein secretion (UcPS) pathway. By employing C. elegans–polarized intestine epithelia, we recently have revealed that the small GTPase RAB-8/Rab8 serves as an important player in the process. Nonetheless, its function and the relevant UcPS itinerary remain poorly understood. Here, we show that deregulated RAB-8 activity resulted in impaired apical UcPS, which increased sensitivity to infection and environmental stress. We also identified the SNARE VTI-1/Vti1a/b as a new RAB-8–interacting factor involved in the apical UcPS. Besides, RAB-11/Rab11 was capable of recruiting RABI-8/Rabin8 to reduce the guanine nucleotide exchange activity of SMGL-1/GEF toward RAB-8, indicating the necessity of a finely tuned RAB-8/RAB-11 network. Populations of RAB-8– and RAB-11–positive endosomal structures containing the apical UcPS cargo moved toward the apical side. In the absence of RAB-11 or its effectors, the cargo was retained in RAB-8– and RAB-11–positive endosomes, respectively, suggesting that these endosomes are utilized as intermediate carriers for the UcPS.
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