Design of an Aptasensor Based on AuNPs@Fc-rGO for Detection of GPC3

Abstract Hepatocellular carcinoma (HCC) is the cancers with a high incidence and mortality rate.. Its existence poses a great threat to people’s life and health. Recently, Glypican-3 (GPC3) has became one of the valuable markers for the diagnosis of HCC. In this study, a GPC3 aptasensor was constructed based on AuNPs@Fc-rGO and the specific recognition of GPC3 aptamer. AuNPs@Fc-rGO had electrochemical activity, signal amplification and biocompatibility. Under the optimized conditions, the value of peak current correlate linearly with the concentration of GPC3 from 0.01 to 10.0 μg/mL, the linear equation was Y = 6.8223-0.3225X with R 2 = 0.9925, and the LOD was 3.16 ng/mL. In addition, the experimental results show that the sensor has excellent stability and specificity. It provides a simple detection method for patients with early HCC.