司他内酯
生物合成
开枪
斯特里加
奥罗班切
酶
发芽
体外
化学
斯特里加·赫蒙蒂卡
产量(工程)
体内
植物
生物化学
生物
生物技术
突变体
材料科学
冶金
基因
拟南芥
作者
Kojiro Kawada,Tatsuo Saito,Satoshi Onoda,Takuma Inayama,Ikuo Takahashi,Yoshiya Seto,T. Nomura,Yasuyuki Sasaki,Tadao Asami,Shunsuke Yajima,Shinsaku Ito
出处
期刊:ACS omega
[American Chemical Society]
日期:2023-04-05
卷期号:8 (15): 13855-13862
被引量:1
标识
DOI:10.1021/acsomega.3c00098
摘要
Strigolactones (SLs), phytohormones that inhibit shoot branching in plants, promote the germination of root-parasitic plants, such as Striga spp. and Orobanche spp., which drastically reduces the crop yield. Therefore, reducing SL production via chemical treatment may increase the crop yield. To design specific inhibitors, it is valid to utilize the substrate structure of the target proteins as lead compounds. In this study, we focused on Os900, a rice enzyme that oxidizes the SL precursor carlactone (CL) to 4-deoxyorobanchol (4DO), and synthesized 10 CL derivatives. The effects of the synthesized CL derivatives on SL biosynthesis were evaluated by the Os900 enzyme assay in vitro and by measuring 4DO levels in rice root exudates. We identified some CL derivatives that inhibited SL biosynthesis in vitro and in vivo.
科研通智能强力驱动
Strongly Powered by AbleSci AI