[Experiment on Jianpi Qingre Huoxue Decoction Drug Serum Regulates SW480 Cell Proliferation,Apoptosis,Cycle and the Expression of β-Catenin by Altering PI3K/Akt Signal Pathway].

To study Jianpi Qingre Huoxue decoction( JPQRHX) in preventing colon cancer by observing SW480 cells proliferation,apoptosis,cycle and the expression of P-β-catenin, and to research its mechanism.SW480 cells were incubated with serum containing blank serum, different concentrations of JPQRHX decocition and PI3 K blocking agent LY294002 for 24 h,respectively. Cell proliferation was detected by MTT assay, cell cycle and apoptosis were detected by flow cytometry. The protein translocation of P-β-catenin was assayed by immunofluorescent staining.The inhibitory rate and apoptosis rate in JPQRHX decoction group were higher than control group( P < 0. 05),respectively. S phase cells were increased significantly, and G1 phase cells and LY294002 group cells were decreased significantly( P < 0. 05). The P-β-catenin in JPQRHX decoction groups were mainly expressed in membrane, while the P-β-catenin in the control group was characterized by deletion in membrane and increased in nucleus.JPQRHX decoction has the ability in curing colon cancer, and the mechanism is associated with altering the expression of P-β-catenin in the cells nucleus, blocking SW480 cells cycle at G1 phase, inhibiting SW480 cells proliferation, and inducing SW480 cell apoptosis.