小檗碱
微泡
医学
脊髓损伤
药理学
巨噬细胞
脊髓
化学
体外
小RNA
生物化学
基因
精神科
作者
Zhanshan Gao,Chuanjie Zhang,Daoyong Li,Jiaquan Lin,Xifan Mei,Chao Wu
出处
期刊:Social Science Research Network
[Social Science Electronic Publishing]
日期:2020-01-01
摘要
Spinal cord injury (SCI) causes immune activation of resident microglia. Activated microglia have two different phenotypes, the pro-inflammatory classically activated (M1) phenotype and the anti-inflammatory alternatively activated (M2) phenotype. M1 phenotype microglia are the key factor in inflammation. The treatment of SCI remains a huge challenge due to the nontargeting and inefficiency of anti-inflammatory drugs through the blood-brain barrier (BBB). The purpose of this experiment was to design M2-type primary peritoneal macrophages exosomes (Exos) as a drug carrier for berberine (Ber), which can be efficiently targeted to deliver drugs to the injured spinal cord due to the natural advantage of Exos across the BBB. The Exos were loaded with Ber by an ultrasonic method. In vitro and in vivo experiments confirmed that the loaded sample (Exos-Ber) could decrease the M1 protein marker iNOS, elevate the M2 protein marker CD206 and reduce inflammatory and apoptotic cytokines (TNF-α, IL-1β, IL-6, Caspase 9, Caspase 8), which showed that Exos-Ber had a good anti-inflammatory and anti-apoptotic effect by inducing microglia from the M1 phenotype to M2 phenotype polarization. Moreover, the motor function of SCI mice was significantly improved after Exos-Ber treatment, indicating that Exos-Ber is a potential agent for SCI therapy.
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