化学
适体
流体学
膜
循环肿瘤细胞
活力测定
生物物理学
纳米技术
生物相容性
生物膜
微流控
细胞
生物化学
分子生物学
癌症
航空航天工程
材料科学
转移
有机化学
工程类
内科学
生物
医学
作者
Lingling Wu,Hong‐ming Ding,Xin Qu,Xiaoyan Shi,Jianmin Yang,Mengjiao Huang,Jialü Zhang,Huimin Zhang,Jia Song,Lin Zhu,Yanling Song,Yu‐qiang Ma,Chaoyong Yang
摘要
The ubiquitous biomembrane interface, with its dynamic lateral fluidity, allows membrane-bound components to rearrange and localize for high-affinity multivalent ligand–receptor interactions in diverse life activities. Inspired by this, we herein engineered a fluidic multivalent nanointerface by decorating a microfluidic chip with aptamer-functionalized leukocyte membrane nanovesicles for high-performance isolation of circulating tumor cells (CTCs). This fluidic biomimetic nanointerface with active recruitment-binding afforded significant affinity enhancement by 4 orders of magnitude, exhibiting 7-fold higher capture efficiency compared to a monovalent aptamer functionalized-chip in blood. Meanwhile, this soft nanointerface inherited the biological benefits of a natural biomembrane, minimizing background blood cell adsorption and maintaining excellent CTC viability (97.6%). Using the chip, CTCs were successfully detected in all cancer patient samples tested (17/17), suggesting the high potential of this fluidity-enhanced multivalent binding strategy in clinical applications. We expect this bioengineered interface strategy will lead to the design of innovative biomimetic platforms in the biomedical field by leveraging natural cell–cell interaction with a natural biomaterial.
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