羊毛甾醇
环蒿醇
生物合成
ATP合酶
生物化学
生物
拟南芥
甾醇
基因
化学
胆固醇
突变体
作者
Masashi Suzuki,Ting Xiang,Kiyoshi Ohyama,Hikaru Seki,Kazuki Saito,Toshiya Muranaka,Hiroaki Hayashi,Yuji Katsube,Toshio Kushiro,Masabumi Shibuya,Yutaka Ebizuka
出处
期刊:Plant and Cell Physiology
[Oxford University Press]
日期:2006-05-01
卷期号:47 (5): 565-571
被引量:109
摘要
Sterols are important as structural components of plasma membranes and precursors of steroidal hormones in both animals and plants. Plant sterols show a wide structural variety and significant structural differences from those of animals. To elucidate the origin of structural diversity in plant sterols, their biosynthesis has been extensively studied [Benveniste (2004) Annu. Rev. Plant. Biol. 55: 429, Schaller (2004) Plant Physiol. Biochem. 42: 465]. The differences in the biosynthesis of sterols between plants and animals begin at the step of cyclization of 2,3-oxidosqualene, which is cyclized to lanosterol in animals and to cycloartenol in plants. However, here we show that plants also have the ability to synthesize lanosterol directly from 2,3-oxidosqualene, which may lead to a new pathway to plant sterols. The Arabidopsis gene At3g45130, designated LAS1, encodes a functional lanosterol synthase in plants. A phylogenetic tree showed that LAS1 belongs to the previously uncharacterized branch of oxidosqualene cyclases, which differs from the cycloartenol synthase branch. Panax PNZ on the same branch was also shown to be a lanosterol synthase in a yeast heterologous expression system. The higher diversity of plant sterols may require two biosynthetic routes in steroidal backbone formation.
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