细胞凋亡
细胞色素c
钙
内质网
线粒体
化学
肌浆
生物
男科
生物化学
医学
有机化学
作者
Bing Guo,Wangang Zhang,R.K. Tume,Nicholas J. Hudson,Feng Huang,Y. Whitney Yin,Guanghong Zhou
出处
期刊:Meat Science
[Elsevier]
日期:2016-05-01
卷期号:115: 34-40
被引量:33
标识
DOI:10.1016/j.meatsci.2016.01.003
摘要
Eight pale, soft and exudative (PSE) and eight reddish-pink, firm and non-exudative (RFN) porcine longissimus muscle samples were selected based on pH and L* at 1h postmortem (PM), and drip loss at 24h PM, and used to evaluate the cellular calcium and apoptosis status. We found that SERCA1 was decreased, while IP3R was decreased in PSE meat (P<0.05), indicative of the overloaded sarcoplasmic calcium status. In PSE meat, the pro-apoptotic factor BAX was increased while the anti-apoptotic factor Bcl-2 was decreased (P<0.05). The significantly increased activity of caspase 3 and the expression of its cleavage fragment suggested higher apoptotic potential in PSE meat compared with RFN meat (P<0.05). Moreover, the significantly higher expression level of cytochrome C (P<0.05) suggests the important role of mitochondria during apoptosis appearance in PSE meat. Taken together, our data inferred that the calcium channel disorder present in PSE meat was associated with the increased apoptotic potential.
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