重组信号序列
重组酶
V(D)J复合
生物
重组激活基因
拉格2
分子生物学
重组
基因重排
非同源性末端接合
遗传学
同源重组
基因
FLP-FRT重组
DNA
遗传重组
免疫球蛋白类转换
位点特异性重组
作者
Marjorie A. Oettinger,David G. Schatz,Claude Gorka,David Baltimore
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1990-06-22
卷期号:248 (4962): 1517-1523
被引量:1365
标识
DOI:10.1126/science.2360047
摘要
The vast repertoire of immunoglobulins and T cell receptors is generated, in part, by V(D)J recombination, a series of genomic rearrangements that occur specifically in developing lymphocytes. The recombination activating gene, RAG-1, which is a gene expressed exclusively in maturing lymphoid cells, was previously isolated. RAG-1 inefficiently induced V(D)J recombinase activity when transfected into fibroblasts, but cotransfection with an adjacent gene, RAG-2, has resulted in at least a 1000-fold increase in the frequency of recombination. The 2.1-kilobase RAG-2 complementary DNA encodes a putative protein of 527 amino acids whose sequence is unrelated to that of RAG-1. Like RAG-1, RAG-2 is conserved between species that carry out V(D)J recombination, and its expression pattern correlates precisely with that of V(D)J recombinase activity. In addition to being located just 8 kilobases apart, these convergently transcribed genes are unusual in that most, if not all, of their coding and 3′ untranslated sequences are contained in single exons. RAG-1 and RAG-2 might activate the expression of the V(D)J recombinase but, more likely, they directly participate in the recombination reaction.
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