生物结合
碳纳米管
表面改性
纳米技术
材料科学
药物输送
聚乙二醇
共轭体系
拉曼光谱
体内
生物传感器
生物物理学
化学
聚合物
有机化学
生物
光学
物理
物理化学
生物技术
复合材料
作者
Zhuang Liu,Scott M. Tabakman,Zhuo Chen,Hongjie Dai
出处
期刊:Nature Protocols
[Springer Nature]
日期:2009-09-01
卷期号:4 (9): 1372-1381
被引量:411
标识
DOI:10.1038/nprot.2009.146
摘要
Biomedical applications of carbon nanotubes have attracted much attention in recent years. Here, we summarize our previously developed protocols for functionalization and bioconjugation of single-walled carbon nanotubes (SWNTs) for various biomedical applications including biological imaging; using nanotubes as Raman, photoluminescence and photoacoustic labels; sensing using nanotubes as Raman tags and drug delivery. Sonication of SWNTs in solutions of phospholipid-polyethylene glycol (PL-PEG) is our most commonly used protocol of SWNT functionalization. Compared with other frequently used covalent strategies, our non-covalent functionalization protocol largely retains the intrinsic optical properties of SWNTs, which are useful in various biological imaging and sensing applications. Functionalized SWNTs are conjugated with targeting ligands, including peptides and antibodies for specific cell labeling in vitro or tumor targeting in vivo. Radio labels are introduced for tracking and imaging of SWNTs in real time in vivo. Moreover, SWNTs can be conjugated with small interfering RNA (siRNA) or loaded with chemotherapy drugs for drug delivery. These procedures take various times ranging from 1 to 5 d.
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