Zeta电位
电泳
粘附
细菌
大肠杆菌
化学
电泳图谱
生物物理学
微生物学
生物
色谱法
纳米技术
生物化学
材料科学
遗传学
有机化学
纳米颗粒
基因
作者
Ewa Kłodzińska,Michał Szumski,Ewelina Dziubakiewicz,Katarzyna Hrynkiewicz,Ewa Skwarek,W. Janusz,Bogusław Buszewski
标识
DOI:10.1002/elps.200900559
摘要
The aggregation and/or adhesion of bacterial cells is a serious disadvantage of electrophoretic separations. In this study, physicochemical surface characteristics of bacteria were measured to establish their role in bacterial adhesion and aggregation on the basis of electrophoretic behavior of different clinical strains of Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria. The number and the shape of peaks obtained on the electropherograms were connected with the zeta potential measurements and in-line microscope observation using specially designed CE fluorescence stereomicroscope setup. These results suggest that the lower the zeta potential, the higher the number of smaller peaks detected. The direct microscopic observation of electrophoretic movement proved the presence of many small aggregates originating from individual or clustered bacterial cells. On the other hand, lower zeta potential was also observed for dead bacterial cells, which suggested that some of the peaks can be attributed to viable cells while the other to the dead ones.
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