亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Longitudinal Genotyping of Candida dubliniensis Isolates Reveals Strain Maintenance, Microevolution, and the Emergence of Itraconazole Resistance

杜氏假丝酵母 基因分型 基因型 生物 病毒学 抗药性 微生物学 伊曲康唑 遗传学 白色体 白色念珠菌 抗真菌 基因
作者
Michael Fleischhacker,Julia Pasligh,Gary P. Moran,Markus Ruhnke
出处
期刊:Journal of Clinical Microbiology [American Society for Microbiology]
卷期号:48 (5): 1643-1650 被引量:19
标识
DOI:10.1128/jcm.01522-09
摘要

We investigated the population structure of 208 Candida dubliniensis isolates obtained from 29 patients (25 human immunodeficiency virus [HIV] positive and 4 HIV negative) as part of a longitudinal study. The isolates were identified as C. dubliniensis by arbitrarily primed PCR (AP-PCR) and then genotyped using the Cd25 probe specific for C. dubliniensis. The majority of the isolates (55 of 58) were unique to individual patients, and more than one genotype was recovered from 15 of 29 patients. A total of 21 HIV-positive patients were sampled on more than one occasion (2 to 36 times). Sequential isolates recovered from these patients were all closely related, as demonstrated by hybridization with Cd25 and genotyping by PCR. Six patients were colonized by the same genotype of C. dubliniensis on repeated sampling, while strains exhibiting altered genotypes were recovered from 15 of 21 patients. The majority of these isolates demonstrated minor genetic alterations, i.e., microevolution, while one patient acquired an unrelated strain. The C. dubliniensis strains could not be separated into genetically distinct groups based on patient viral load, CD4 cell count, or oropharyngeal candidosis. However, C. dubliniensis isolates obtained from HIV-positive patients were more closely related than those recovered from HIV-negative patients. Approximately 8% (16 of 194) of isolates exhibited itraconazole resistance. Cross-resistance to fluconazole was only observed in one of these patients. Two patients harboring itraconazole-resistant isolates had not received any previous azole therapy. In conclusion, longitudinal genotyping of C. dubliniensis isolates from HIV-infected patients reveals that isolates from the same patient are generally closely related and may undergo microevolution. In addition, isolates may acquire itraconazole resistance, even in the absence of prior azole therapy.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
风华正茂完成签到,获得积分10
21秒前
32秒前
41秒前
jimmy_bytheway完成签到,获得积分0
42秒前
桃桃发布了新的文献求助10
45秒前
可爱的函函应助桃桃采纳,获得10
53秒前
1分钟前
1分钟前
1分钟前
1分钟前
NexusExplorer应助科研通管家采纳,获得10
1分钟前
所所应助爱笑的毛衣采纳,获得10
1分钟前
1分钟前
2分钟前
duan完成签到 ,获得积分10
2分钟前
holder完成签到,获得积分10
2分钟前
2分钟前
沐白发布了新的文献求助10
2分钟前
2分钟前
刘宇童发布了新的文献求助10
2分钟前
大模型应助吕易巧采纳,获得10
2分钟前
迷人问兰完成签到,获得积分10
2分钟前
闪闪映易完成签到 ,获得积分10
2分钟前
2分钟前
吕易巧发布了新的文献求助10
3分钟前
吕易巧完成签到,获得积分10
3分钟前
3分钟前
Liiiiiiiiii发布了新的文献求助10
3分钟前
XuchaoD完成签到,获得积分10
3分钟前
3分钟前
今后应助Liiiiiiiiii采纳,获得10
3分钟前
顾矜应助科研通管家采纳,获得10
3分钟前
Akim应助科研通管家采纳,获得10
3分钟前
3分钟前
3分钟前
4分钟前
4分钟前
小张完成签到 ,获得积分10
4分钟前
可耐的冰萍完成签到,获得积分10
4分钟前
充电宝应助干净涵梅采纳,获得10
4分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990049
求助须知:如何正确求助?哪些是违规求助? 3532108
关于积分的说明 11256354
捐赠科研通 3270976
什么是DOI,文献DOI怎么找? 1805166
邀请新用户注册赠送积分活动 882270
科研通“疑难数据库(出版商)”最低求助积分说明 809228