Longitudinal Genotyping of Candida dubliniensis Isolates Reveals Strain Maintenance, Microevolution, and the Emergence of Itraconazole Resistance

杜氏假丝酵母 基因分型 基因型 生物 病毒学 抗药性 微生物学 伊曲康唑 遗传学 白色体 白色念珠菌 抗真菌 基因
作者
Michael Fleischhacker,Julia Pasligh,Gary P. Moran,Markus Ruhnke
出处
期刊:Journal of Clinical Microbiology [American Society for Microbiology]
卷期号:48 (5): 1643-1650 被引量:19
标识
DOI:10.1128/jcm.01522-09
摘要

We investigated the population structure of 208 Candida dubliniensis isolates obtained from 29 patients (25 human immunodeficiency virus [HIV] positive and 4 HIV negative) as part of a longitudinal study. The isolates were identified as C. dubliniensis by arbitrarily primed PCR (AP-PCR) and then genotyped using the Cd25 probe specific for C. dubliniensis. The majority of the isolates (55 of 58) were unique to individual patients, and more than one genotype was recovered from 15 of 29 patients. A total of 21 HIV-positive patients were sampled on more than one occasion (2 to 36 times). Sequential isolates recovered from these patients were all closely related, as demonstrated by hybridization with Cd25 and genotyping by PCR. Six patients were colonized by the same genotype of C. dubliniensis on repeated sampling, while strains exhibiting altered genotypes were recovered from 15 of 21 patients. The majority of these isolates demonstrated minor genetic alterations, i.e., microevolution, while one patient acquired an unrelated strain. The C. dubliniensis strains could not be separated into genetically distinct groups based on patient viral load, CD4 cell count, or oropharyngeal candidosis. However, C. dubliniensis isolates obtained from HIV-positive patients were more closely related than those recovered from HIV-negative patients. Approximately 8% (16 of 194) of isolates exhibited itraconazole resistance. Cross-resistance to fluconazole was only observed in one of these patients. Two patients harboring itraconazole-resistant isolates had not received any previous azole therapy. In conclusion, longitudinal genotyping of C. dubliniensis isolates from HIV-infected patients reveals that isolates from the same patient are generally closely related and may undergo microevolution. In addition, isolates may acquire itraconazole resistance, even in the absence of prior azole therapy.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
朽木完成签到 ,获得积分10
1秒前
1秒前
fanyueyue应助111采纳,获得10
3秒前
3秒前
3秒前
kcmat发布了新的文献求助10
4秒前
hh完成签到,获得积分10
5秒前
Philadelphus发布了新的文献求助10
6秒前
einuo完成签到,获得积分10
6秒前
AKYDXS完成签到,获得积分10
9秒前
昏睡的蟠桃应助Llllll采纳,获得200
9秒前
科研通AI2S应助hao采纳,获得10
9秒前
10秒前
10秒前
香蕉觅云应助阿湫采纳,获得10
11秒前
星辰大海应助星辰采纳,获得10
11秒前
阿卡宁完成签到,获得积分10
11秒前
lzw完成签到 ,获得积分10
11秒前
沉静烧仙草完成签到,获得积分20
12秒前
烟花应助嘉嘉琦采纳,获得10
12秒前
隐形曼青应助科研通管家采纳,获得10
12秒前
Hello应助科研通管家采纳,获得10
13秒前
Ava应助科研通管家采纳,获得10
13秒前
上官若男应助科研通管家采纳,获得10
13秒前
13秒前
赘婿应助科研通管家采纳,获得10
13秒前
烟花应助科研通管家采纳,获得10
13秒前
FashionBoy应助科研通管家采纳,获得10
13秒前
英俊的铭应助科研通管家采纳,获得10
13秒前
在水一方应助科研通管家采纳,获得10
13秒前
accepted应助科研通管家采纳,获得10
13秒前
脑洞疼应助科研通管家采纳,获得10
13秒前
13秒前
cdh1994应助kcmat采纳,获得10
13秒前
我是老大应助科研通管家采纳,获得10
13秒前
乐乐应助科研通管家采纳,获得10
13秒前
FashionBoy应助科研通管家采纳,获得10
13秒前
13秒前
上官若男应助科研通管家采纳,获得10
13秒前
13秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038524
求助须知:如何正确求助?哪些是违规求助? 3576221
关于积分的说明 11374737
捐赠科研通 3305912
什么是DOI,文献DOI怎么找? 1819354
邀请新用户注册赠送积分活动 892688
科研通“疑难数据库(出版商)”最低求助积分说明 815048