质粒
生物
基因
重组酶
肠沙门氏菌
遗传学
微生物学
噬菌体
多重耐药
沙门氏菌
抗生素耐药性
安培电阻
细菌
抗生素
重组
大肠杆菌
作者
Benoît Doublet,Grégory Douard,Hayette Targant,Danièle Meunier,Jean-Yves Madec,Axel Cloeckaert
标识
DOI:10.1016/j.mimet.2008.06.010
摘要
The Red recombinase system of bacteriophage Lambda has been used to inactivate chromosomal genes in bacteria using PCR products. In this study, we describe the replacement of the ampicillin resistance marker of helper plasmids pKD46 and pCP20 by a gentamicin resistance gene to disrupt chromosomal genes and then to eliminate FRT flanked resistance gene in multiple antibiotic-resistant Salmonella enterica strains.
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