Photobleaching: Improving the Sensitivity of Fluorescence-Based Immunoassays by Photobleaching the Autofluorescence of Magnetic Beads (Small 3/2019)

In fluorescence-based assays, the autofluorescence of capture surfaces produces strong background noise. In article number 1803751, Amos Danielli and co-workers use photobleaching as a method to reduce the autofluorescence of magnetic beads, a commonly used capture surface. The technique yields a 3-fold improvement in the limit of detection and signal-to-noise ratio of a fluorescence-based immunoassay.