Internuclear Transfer of Genetic Information in kar1-1/KAR1 Heterokaryons in Saccharomyces cerevisiae

异核体 生物 倍性 遗传学 染色体 核心 酿酒酵母 染色体交叉 基因型 基因 细胞生物学 突变体
作者
Susan K. Dutcher
出处
期刊:Molecular and Cellular Biology [Taylor & Francis]
卷期号:1 (3): 245-253 被引量:9
标识
DOI:10.1128/mcb.1.3.245-253.1981
摘要

Heterokaryons of Saccharomyces cerevisiae have been constructed utilizing the kar1-1 mutation, which prevents nuclear fusion during conjugation (J. Conde and G. Fink, Proc. Natl. Acad. Sci. U.S.A. 73:3651-3655, 1976). Each heterokaryon contained two haploid nuclei that were marked on several chromosomes. They segregated haploid progeny (cytoductants), most of which have the nuclear genotype of one or the other of the heterokaryon parents, but they occasionally segregated progeny having a recombinant genotype (exceptional cytoductants). Exceptional cytoductants receive the majority of their genome from one parent (the recipient) and a minority from the other (the donor). Transfer of two markers from the donor nucleus to the recipient is rarely coincident for markers located on different chromosomes but is nearly always coincident for those markers located on the same chromosome, suggesting that whole chromosomes are transferred from the donor nucleus to the recipient. In crosses of kar1-1 X KAR1 parents, either nucleus may act as a recipient or donor with equal probability. Recipient nuclei acquired 9 of the 10 chromosomes examined, with frequencies which were inversely correlated with the size of the chromosome. When a chromosome is acquired by the recipient nucleus, it either replaces its homolog or exists in a disomic condition. Haploid progeny emanating from kar1 X KAR1 crosses are frequently inviable. I tested whether this inviability might be the result of chromosome loss by donor nuclei. Viability of progeny from kar1 X KAR1 heterokaryons was improved when the parental nuclei were diploid to an extent consistent with the hypothesis, and diploid progeny which had become monosomic were recovered from these heterokaryons. The following sequence of events accounts for chromosome transfer in kar1 X KAR1 heterokaryons. After cell fusion, each nucleus in the heterokaryon has a probability of about 0.38 of losing one or more chromosomes. A nucleus sustaining such a loss can become a donor in a chromosome transfer event. If the other nucleus does not sustain a mortal chromosome loss, it can become a recipient in a transfer event. The chance of acquiring a chromosome lost by the donor is greater for smaller chromosomes than for larger ones and is about 0.05 for the average chromosome.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
2秒前
HJJHJH发布了新的文献求助50
3秒前
JW完成签到,获得积分10
3秒前
wanci应助张参采纳,获得10
4秒前
谦让的西装完成签到 ,获得积分10
5秒前
李演员完成签到,获得积分10
6秒前
fei菲飞完成签到,获得积分10
6秒前
8秒前
Zhaowx完成签到,获得积分10
8秒前
Theprisoners完成签到,获得积分0
8秒前
木子发布了新的文献求助30
8秒前
8秒前
下课了吧完成签到,获得积分10
9秒前
丘比特应助xialuoke采纳,获得10
10秒前
zgt01发布了新的文献求助10
12秒前
linfordlu完成签到,获得积分0
12秒前
清浅发布了新的文献求助10
13秒前
风趣的涵柏完成签到,获得积分10
14秒前
16秒前
Chen完成签到 ,获得积分10
17秒前
18秒前
木樨完成签到,获得积分10
19秒前
科研顺利完成签到,获得积分10
20秒前
Bin完成签到,获得积分10
21秒前
gszy1975发布了新的文献求助10
21秒前
十曰发布了新的文献求助10
22秒前
23秒前
23秒前
jiaolulu发布了新的文献求助10
28秒前
畅快的念烟完成签到,获得积分10
30秒前
丽莫莫完成签到,获得积分10
30秒前
钙离子完成签到,获得积分10
31秒前
情怀应助OLDBLOW采纳,获得10
32秒前
潇洒日记本完成签到,获得积分10
33秒前
vsvsgo发布了新的文献求助30
34秒前
量子星尘发布了新的文献求助10
34秒前
xiaodong完成签到,获得积分10
34秒前
34秒前
竞鹤完成签到,获得积分10
34秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038235
求助须知:如何正确求助?哪些是违规求助? 3575992
关于积分的说明 11374009
捐赠科研通 3305760
什么是DOI,文献DOI怎么找? 1819276
邀请新用户注册赠送积分活动 892662
科研通“疑难数据库(出版商)”最低求助积分说明 815022