化学
牛血清白蛋白
高良姜素
白杨素
圆二色性
黄芩素
牡荆素
杨梅素
木犀草素
类黄酮
结合位点
猝灭(荧光)
荧光
异牡荆素
生物化学
抗氧化剂
山奈酚
药理学
物理
医学
量子力学
作者
Jie Zhao,Lin Huang,Renjie Li,Zhuangwei Zhang,Jin Chen,Hongjin Tang
出处
期刊:Food Chemistry
[Elsevier]
日期:2022-03-08
卷期号:385: 132656-132656
被引量:16
标识
DOI:10.1016/j.foodchem.2022.132656
摘要
Bovine serum albumin (BSA) has the potential application of establishing a delivery system for flavonoids. However, the effect of copper on the binding of flavonoids with BSA is unclear. Therefore, the binding of six flavonoids with BSA containing Cu2+ was investigated using UV-vis, fluorescence, and molecular docking. The UV-vis spectral changes demonstrated the formation of flavonoid-Cu2+ complexes. The fluorescence quenching results suggested that the chelation of Cu2+ increased the binding affinity of galangin and baicalin to the BSA but decreased the binding capacity of chrysin, baicalein, luteolin, and vitexin. Synchronous fluorescence data revealed that Cu2+ could influence the secondary structure conformation of BSA binding with flavonoids, which was further confirmed by ANS-binding fluorescence, circular dichroism, and molecular docking. These findings demonstrate that the complexation of Cu2+ significantly affects the binding of flavonoids with BSA, which provides the theoretical basis for the development of natural product-metal complex functional foods.
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