纳米粒子跟踪分析
超离心机
分离(微生物学)
分析超速离心
细胞外小泡
流式细胞术
色谱法
盐析
化学
动态光散射
胞外囊泡
细胞生物学
计算生物学
微泡
生物化学
生物
纳米技术
分子生物学
材料科学
纳米颗粒
生物信息学
小RNA
物理化学
水溶液
基因
作者
Simona Serratì,Antonio J. Palazzo,Annamaria Lapenna,Helena Mateos,Antonia Mallardi,René Massimiliano Marsano,Alessandra Quarta,Mario Del Rosso,Amalia Azzariti
出处
期刊:Biomolecules
[MDPI AG]
日期:2021-12-10
卷期号:11 (12): 1857-1857
被引量:3
摘要
The role of extracellular vesicles (EVs) has been completely re-evaluated in the recent decades, and EVs are currently considered to be among the main players in intercellular communication. Beyond their functional aspects, there is strong interest in the development of faster and less expensive isolation protocols that are as reliable for post-isolation characterisations as already-established methods. Therefore, the identification of easy and accessible EV isolation techniques with a low price/performance ratio is of paramount importance. We isolated EVs from a wide spectrum of samples of biological and clinical interest by choosing two isolation techniques, based on their wide use and affordability: ultracentrifugation and salting-out. We collected EVs from human cancer and healthy cell culture media, yeast, bacteria and Drosophila culture media and human fluids (plasma, urine and saliva). The size distribution and concentration of EVs were measured by nanoparticle tracking analysis and dynamic light scattering, and protein depletion was measured by a colorimetric nanoplasmonic assay. Finally, the EVs were characterised by flow cytometry. Our results showed that the salting-out method had a good efficiency in EV separation and was more efficient in protein depletion than ultracentrifugation. Thus, salting-out may represent a good alternative to ultracentrifugation.
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