荧光团
化学
细胞内
酶
荧光
活性氧
人工酶
催化作用
生物相容性
生物物理学
酯酶
生物化学
有机化学
生物
物理
量子力学
作者
Yufei Di,Endong Zhang,Zhiwen Yang,Qi Shen,Xuancheng Fu,Gang Song,Chuankun Zhu,Haotian Bai,Yiming Huang,Fengting Lv,Libing Liu,Shu Wang
标识
DOI:10.1002/anie.202116457
摘要
Inside living cells, regulation of catalytic activity of artificial enzymes remains challenging due to issues such as biocompatibility, efficiency, and stability of the catalyst, by which the practical applications of artificial enzymes have been severely hindered. Here, an artificial enzyme, PTT-SGH, with responsiveness to reactive oxygen species (ROS), was obtained by introducing a catalytic histidine residue to pentaerythritol tetra(3-mercaptopropionate) (PTT). The artificial enzyme formed large aggregates in cells via the intracellular ROS-mediated oxidation of thiol groups. The process was significantly facilitated in tumor cells because of the higher ROS concentration in the tumor microenvironment. The catalytic activity of this artificial enzyme was intensively enhanced through deprotonation of cross-linked PTT-SGH, which showed typical esterase activities. Selective fluorescence imaging of tumor cells was achieved using the artificial enzyme to trigger the cleavage of the ester bond of the caged fluorophore inside living cells.
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