秀丽隐杆线虫
RNA干扰
生物
模式生物
细胞分裂
细胞生物学
有丝分裂
活体细胞成像
功能(生物学)
细胞
减数分裂
免疫荧光
核糖核酸
遗传学
基因
抗体
作者
Ian D. Wolff,Nikita S. Divekar,Sarah M. Wignall
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 19-35
被引量:3
标识
DOI:10.1007/978-1-0716-1904-9_2
摘要
The nematode Caenorhabditis elegans is a widely used model organism for the study of mitotic and meiotic cell division. These self-fertilizing worms are particularly advantageous for such studies because they rapidly reproduce (each worm lays ~250 eggs in only 3-4 days) and the cell division machinery is highly conserved between worms and humans. Worms are also genetically tractable and proteins can be readily depleted using RNA interference (RNAi), allowing for the characterization of protein function in vivo. To assess phenotypes, spindles can be directly visualized within the worm using fluorescent protein tags or embryos can be dissected out of the worm and immunostained. A combination of these techniques allows comprehensive characterization of a protein's function in a relatively short time span. Here, we describe methods for each of these techniques: RNA interference through feeding, in utero live imaging, in utero fixed imaging, and immunofluorescence.
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