Poly(hexamethylene biguanide) hydrochloride (PHMB) loaded poly(vinyl alcohol) (PVA) fibrous films were produced via electrospinning as active food packaging material. Fourier transform infrared spectroscopy analysis confirmed that PHMB has been incorporated into the PVA fibers and scanning electron microscopy analysis indicated that the fibrous films were successfully prepared. The thermal stability and antibacterial activity of PVA/PHMB fibrous films were tested. The application of fibrous films on the preservation of Japanese sea bass ( Lateolabrax japonicus ) during cold storage at 4 °C was investigated. Compared with the control sample, PHMB loaded PVA fibrous films effectively lowered the total viable count, inhibited Pseudomonas spp. and H 2 S-producing bacteria, as well as delayed the production of total volatile basic nitrogen. The results revealed that PVA/PHMB fibrous films had a positive effect on maintaining quality and extending the shelf life of sea bass. • Fibrous films loaded with antibacterial agent were prepared by electrospinning. • The fibrous films had excellent antibacterial properties. • The shelf life of Japanese sea bass fillets was extended by the fibrous films.