次氯酸
次氯酸盐
荧光
化学
荧光团
部分
内生
过氧化氢
检出限
吩噻嗪
光化学
斑马鱼
荧光寿命成像显微镜
生物物理学
光毒性
活体细胞成像
体内
荧光显微镜
生物化学
立体化学
色谱法
无机化学
物理
基因
药理学
生物
医学
量子力学
作者
Hongxia Cui,Peipei Hou,Yu Liu,Jingwen Sun,Haiyan Zhang,Yan Zheng,Qi Liu,Song Chen
标识
DOI:10.1016/j.jphotochem.2021.113343
摘要
Hypochlorite (ClO−), as a typical weak acidic ROS, is mainly endogenously produced by myeloperoxidase (MPO) catalyzing hydrogen peroxide (H2O2) and chloride ion (Cl−) in leukocytes. Imbalance of hypochlorite concentration will not only cause biomolecular oxidation in organism, but also are correlated with a variety of physiological diseases. In this study, a novel ratiometric fluorescence probe PHPQ for detecting ClO- by incorporating the phenothiazine moiety into the HPQ fluorophore was designed and synthesized. The probe PHPQ was shown to be capable of selectively and sensitively monitoring ClO- in a fine ratiometric manner. It also displayed the merits of large blue-shift (98 nm), rapid response (30 s), excellent sensitivity (15 nM) and significant fluorescence ratiometric variations (253-fold). Moreover, PHPQ was successfully utilized for bioimaging exogenous/endogenous ClO- in living cells (MGC-803 and RAW264.7) and in zebrafish.
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