色谱法
衍生化
化学
气相色谱法
质谱法
气相色谱-质谱法
转甲基
氨基酸
生物化学
蛋氨酸
作者
Mathias Brands,Philipp Gutbrod,Peter Dörmann
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 43-57
被引量:5
标识
DOI:10.1007/978-1-0716-1362-7_4
摘要
Gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) represent powerful tools for the quantitative and structural analysis of plant lipids. Here, we outline protocols for the isolation, separation, and derivatization of plant lipids for subsequent GC and GC-MS analysis. Plant lipids are extracted with organic solvents and separated according to their polarity by thin-layer chromatography or solid phase extraction. As most lipids are not volatile, the analytes are derivatized by transmethylation or trimethylsilylation to enable the transition of the molecules into the gas phase. After separation on the polymer matrix of the GC column, the analytes are detected by flame ionization or mass spectrometry. This chapter includes methods suitable for the analysis of lipid-bound or free fatty acids, long chain alcohols, and monoacylglycerols and for the determination of double bond positions in fatty acids.
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